Laboratoire Francis PERRIN
URA CNRS-CEA 2453
This website is no more mainteined. See LIDYL or NIMBE
The GOUTTELIUM setup : an IR spectroscopy in a cold environment
Last Update: 29 July 2014

Etude du photorécepteur "Photoactive Yellow Protein" par dichroïsme circulaire femtoseconde
Last Update: 21 February 2016

L'interaction médicament-protéine étudié par spectroscopie de fluorescence résolue en temps
Last Update: 9 December 2013

"Cœur silicium - coquille carbone" pour batteries Li-Ion
Last Update: 6 October 2015

Dynamic of peptids in gas phase and photostability of proteins
Last Update: 21 May 2013

A method to observe the complete vibrational spectrum of ionized molecules
Last Update: 21 May 2013

The strength of the NHamide---Smethionine revealed by spectroscopy of small peptides

The description of the interactions controlling the shape of a protein is crucial in understanding the cellular mechanisms, but is still difficult to achieve on biological systems because of their complexity.

In this context, the use of model molecules makes accessible to experiments many biological problems lying at the heart of current societal issues. Gas-phase IR/UV spectroscopy of small peptides is an outstanding example. The study of peptides containing the methionine residue has recently shown that NHamide---Smethionine hydrogen bonds are particularly strong. They are, for example, as strong as the "classical" NHamide---OCamide hydrogen bonds which define the secondary structure of proteins.

Analysis of protein structures identified to date reveals that the type of NHamide---Smethionine bond observed experimentally occurs on 12% of methionines. Comparison of the parameters defining the NHamide---Smethionine bond shows that the hydrogen bonds formed in the gas phase faithfully reproduce those observed in proteins. This strongly suggests that the properties of the NHamide---Smethionine bonds highlighted in the gas phase, especially their strength, are identical to those naturally occurring in proteins.

The study of the constraints imposed to the backbone by these NHamide---Smethionine bonds shows that they reduce the range of possible values ​​for the Ramachandran angles. This phenomenon common to gas-phase peptides and proteins having these NHamide---Smethionine bonds thus illustrates the effect that these bonds can have on the rigidity of the peptide backbone. These results already open interesting paths in understanding the way antitumor drugs act.  Journal of Physical Chemistry Letters 2012, 3, 755−759

 
NH<sub>amide</sub>---S<sub>méthionine</sub>---> bond revealed in a dipeptide

NHamide---Sméthionine---> bond revealed in a dipeptide

Michel MONS - 01 69 08 20 01

Eric GLOAGUEN - 01 69 08 35 82

 

Imagerie photochimique du champ proche optique de nanocubes d’or
Last Update: 15 March 2012

Des tapis de nanotubes alignés, en grande surface !
Last Update: 31 January 2012

Nouvelles lumières sur l’interaction entre les rayons UVA et l’ADN
Last Update: 3 February 2014

Synergy btetween experiment and theory for the simulation of protein folding
Last Update: 21 September 2010

Un nouveau composé aux propriétés intéressantes d'absorption optique dans le domaine visible : TiO
Last Update: 17 June 2010

Synthèse de nanotubes marqués au carbone 14 pour des études de biodistribution
Last Update: 13 January 2011

A surprising dynamic of cluster fragmentation
Last Update: 13 November 2007

Chirality and folding of peptide chains
Last Update: 23 March 2007

Recent results in physicochemistry with the very new laser PLFA
Last Update: 16 March 2007

Effets coopératifs dans l'absorption du rayonnement UV par les bases de l'ADN
Last Update: 3 February 2014

Repliement de chaînes peptides en détente supersonique
Last Update: 3 February 2014

Formation of carbon nanotube multi-layers and identification of their growth mode
Last Update: 5 January 2010

Participation d'un état de Rydberg à la redistribution ultrarapide de l'énergie électronique du TDMAE [tetrakis(diméthylamino)éthylene]
Last Update: 3 February 2014